By J. B. Clarke, U. Kreuzberg-Duffy, C. MacDonald, H. Moulsdale, J. Golding, B. Griffiths (auth.), E. C. Beuvery, J. B. Griffiths, W. P. Zeijlemaker (eds.)
Animal mobilephone expertise is a self-discipline of becoming significance, which goals no longer basically at knowing constitution, functionality and behavior of differentiated animal cells, yet specifically on the improvement in their talents helpful for scientific software. themes of curiosity during this regard contain: viral vaccines, pharmaceutical proteins and novel functions comparable to gene treatment and organ tradition.
definitely, those complaints of the joint assembly of the ecu Society for Animal mobile know-how and the japanese organization for Animal mobilephone know-how (Veldhoven, The Netherlands, September 1994) evaluate the newest prestige of the sector, and should be most beneficial to someone actively interested by the tradition of animal cells and its functions. The contributions to this quantity have been strictly chosen at the foundation of caliber and novelty of contents.
Kluwer is honoured for you to upload this paintings to its strongly constructing booklet programme in mobilephone and tissue tradition, which now has its connections to all significant Societies during this box world wide.
Audience: telephone biologists, biochemists, molecular biologists, immunologists, virologists and all different disciplines regarding animal mobilephone expertise, operating in an instructional setting, in addition to in (biotechnology or pharmaceutical) industry.
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Additional info for Animal Cell Technology: Developments Towards the 21st Century
The cells in this state should be kept over a long period of fermentation, preferably without proliferation. We have tried to find a method by which proliferation can be differentially Introduction Most cell lines that are used for the production of recombinant proteins proliferate spontaneously at a high rate. In many types of cultivation systems these cells still keep growing after having reached the desired cell density. Further proliferation leads to cell death as a consequence of nutrition and oxygen depletion as well as of accumulation of lactate and toxic products.
This PCR product M6 encodes an IRF-l deletion mutant missing the 34 N-terrninal amino acids. The resulting fragment was cloned into pTBCl. The coding region of the murine IRF-l cDNA gene inserted into pMPSVHE. pM17 -hER was generated by integrating the insert of plasmid HE14 into the MCS site of pM17HE. , 1986). pM17 -IRF-l-hER was constructed by inserting the fragment encoding IRF-l with additional proline residues and the hER part into pMTIHE. , 1989) was created. pAI5-TKCAT contains a synthetic promoter based on the Herpes Simplex Virus TK promoter which is complemented with 5 repeats of an IRF-l binding sequence.
Establishment and Characterization of a Stable, Amplified Chinese Hamster Ovary (CHO) Cell Line Producing human Insulin-like Growth Factor Binding Protein-I (hIGFBP-I) C. DYRlNG#*, K. MELLSTROM' and M. LAKE# :Pharmacia Bioscience Center, S-112 87 Stockholm, Sweden and the Technical University of Denmark, DK-2800 Lyngby, Denmark I. Introduction Stable expressioo of human Insulin-like growth factor binding protein-l (lGFBP-l), as determined by a biosensor, the BIACore, at high levels has been achieved in Chinese Hamster Ovary (CHO) cells by cotransfection and subsequent coamplification of expression vectors containing the IGFBP-l cDNA and a dihydrofolate reductase (DHFR) cDNA gene into CHO DHFR-deficient cells.
Animal Cell Technology: Developments Towards the 21st Century by J. B. Clarke, U. Kreuzberg-Duffy, C. MacDonald, H. Moulsdale, J. Golding, B. Griffiths (auth.), E. C. Beuvery, J. B. Griffiths, W. P. Zeijlemaker (eds.)